Reads DNA Strider, Fasta, Genbank and EMBL filesħ. Shows translation, Tm, %GC, ORF of selected DNA in real-timeĦ. Highlights text using pre-defined and custom feature librariesĥ. Accurately reflects Dam/Dcm blocking of enzyme sitesĤ. Highlights restriction sites in the editing windowģ. ApE was developed to be a small, easy-to-use plasmid editor.ġ.

Return to DNA Sequencing Core Facilities main page.ApE is a small, easy-to-use plasmid editor. If you have any suggestions for additions or edits to this page, please contact the facility. Sequence Analysis Sofware from ABI to edit and view both analyzed and raw sequence data, clear range at 5’ and 3’ ends, QVs and QV bars for the sequences, and export traces in multiple formats including pdf, phd.1, seq etc.ApE-A Plasmid Editor, to view, edit, and analyze data, including alignment of multiple sequences.Finch TV to view, edit, print, and blast your sequence data.ThermoFisher Connect online Data Analysis Apps.Generally we provide ABI and Fasta files, but if clients require other type of the sequence files, we can re-analyze data, and then send phred or chromatogram files. Standard chromatogram format (.scf) files.Multiple formats of output sequence files:.

Clear range is the region of a DNA sequence that remains after excluding the low quality or error-prone sequence at both 5’and 3’ ends.

The KB basecaller generates QVs from 1 to 99, and the QVs are optionally displayed as bars above each base in the Sequences and Electropherograms views.

QV = -10 log 10 (Pe)Where Pe is the probability of error.

Generation of quality values (QV) to provide basecall accuracy information for pure and mixed base calls.

A novel basecaller algorithm that performs basecalling for pure and mixed base calls.

Notes on analysis of results with Sequence Analysis Software V7.0

Other purification kits may be suitable-please contact the facility to discuss.

Sequencing reaction protocol for BigDye: BigDye Terminator v3.1 Cycle Sequencing Kit Protocol (Applied Biosystems protocol).

Following is some information on the system: The facility DNA sequencing system was upgraded in January 2021 from the ABI 3130xl to the ABI 3500xl.

Ape plasmid editor